Article
Gene 1.7 of bacteriophage T7 confers sensitivity of phage growth to dideoxythymidine.
Proceedings of the National Academy of Sciences of the United States of America - 8 Jul 2008
Tran Ngoc Q, Rezende Lisa F, Qimron Udi, Richardson Charles C, Tabor Stanley
Abstract excerpt
Bacteriophage T7 DNA polymerase efficiently incorporates dideoxynucleotides into DNA, resulting in chain termination. Dideoxythymidine (ddT) present in the medium at levels not toxic to Escherichia coli inhibits phage T7. We isolated 95 T7 phage mutants that were resistant to ddT. All contained a mutation in T7 gene 1.7, a nonessential gene of unknown function. When gene 1.7 was expressed from a plasmid, T7 phage...
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