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Article

A mutant T7 RNA polymerase that is defective in RNA binding and blocked in the early stages of transcription.

Journal of molecular biology - 24 Jan 1997

He B, Rong M, Durbin R K, McAllister W T

Abstract excerpt

We have identified a mutation (E148A) in T7 RNA polymerase (RNAP) that results in an enzyme which aborts transcription primarily when the nascent RNA achieves a length of 5 nt. This phenomenon is observed at a consensus promoter, but is even more strongly observed at promoters that are altered in the initiation region. Although the abortive product is of a fixed length (5 nt), the positions of the base...

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Source

Identifiers

PMID: 9018042
DOI: 10.1006/jmbi.1996.0741

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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