Article
A mutant T7 RNA polymerase that is defective in RNA binding and blocked in the early stages of transcription.
Journal of molecular biology - 24 Jan 1997
He B, Rong M, Durbin R K, McAllister W T
Abstract excerpt
We have identified a mutation (E148A) in T7 RNA polymerase (RNAP) that results in an enzyme which aborts transcription primarily when the nascent RNA achieves a length of 5 nt. This phenomenon is observed at a consensus promoter, but is even more strongly observed at promoters that are altered in...
Topics
- Amino Acids
- Base Sequence
- Binding Sites
- DNA-Directed RNA Polymerases
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Promoter Regions, Genetic
- Protein Conformation
- RNA
- Transcription, Genetic
- Viral Proteins