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Article

Mutants of T7 RNA polymerase that are able to synthesize both RNA and DNA.

FEBS letters - 7 Aug 1995

Kostyuk D A, Dragan S M, Lyakhov D L, Rechinsky V O, Tunitskaya V L, Chernov B K, Kochetkov S N

Abstract excerpt

A mutant T7 RNA polymerase (T7 RNAP) having two amino-acid substitutions (Y639F and S641A) is altered in its specificity towards nucleotide substrates, but is not affected in the specificity of its interaction with promoter and terminator sequences. The mutant enzyme gains the ability to utilize dNTPs and catalyze RNA and DNA synthesis from circular supercoiled plasmid DNA. DNA synthesis can also be initiated...

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PMID: 7544291
DOI: 10.1016/0014-5793(95)00732-o

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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