Article
Mutants of T7 RNA polymerase that are able to synthesize both RNA and DNA.
FEBS letters - 7 Aug 1995
Kostyuk D A, Dragan S M, Lyakhov D L, Rechinsky V O, Tunitskaya V L, Chernov B K, Kochetkov S N
Abstract excerpt
A mutant T7 RNA polymerase (T7 RNAP) having two amino-acid substitutions (Y639F and S641A) is altered in its specificity towards nucleotide substrates, but is not affected in the specificity of its interaction with promoter and terminator sequences. The mutant enzyme gains the ability to utilize...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA
- DNA, Single-Stranded
- DNA, Superhelical
- DNA-Directed DNA Polymerase
- DNA-Directed RNA Polymerases
- Molecular Sequence Data
- Mutation
- Nucleotides
- Plasmids
- RNA
- Substrate Specificity
- Viral Proteins