Article
Mutant bacteriophage T7 RNA polymerases with altered termination properties.
Journal of molecular biology - 30 May 1997
Lyakhov D L, He B, Zhang X, Studier F W, Dunn J J, McAllister W T
Abstract excerpt
We have identified mutants of bacteriophage T7 RNA polymerase (RNAP) that are altered in their ability to pause or terminate at a variety of signals. These signals include a terminator found fortuitously in the human preproparathyroid hormone (PTH) gene, a pause site found in the concatamer junct...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA-Directed RNA Polymerases
- Molecular Sequence Data
- Muramidase
- Mutation
- Parathyroid Hormone
- Peptide Chain Elongation, Translational
- Protein Precursors
- Protein Processing, Post-Translational
- RNA Processing, Post-Transcriptional
- Repetitive Sequences, Nucleic Acid
- Sequence Deletion
- Sequence Homology, Amino Acid