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Article

Isolation of transcriptionally active mutants of T7 RNA polymerase that do not support phage growth.

Journal of molecular biology - 7 Jul 1995

Zhang X, Studier F W

Abstract excerpt

Mutants of bacteriophage T7 RNA polymerase defective in functions other than transcription were sought by random chemical mutagenesis of the cloned gene and selection for inability to support the growth of a T7 mutant whose growth is dependent on T7 RNA polymerase supplied by the host cell. About...

Topics

Amino Acid Sequence
Bacteriophage T7
DNA Mutational Analysis
DNA Replication
DNA-Directed RNA Polymerases
Gene Expression
Genes, Lethal
Genes, Viral
Molecular Sequence Data
Mutation
N-Acetylmuramoyl-L-alanine Amidase
Recombination, Genetic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Suppression, Genetic

Transcription, Genetic

Viral Proteins

Virus Replication

Source

Identifiers

PMID: 7608967
DOI: 10.1006/jmbi.1995.0367

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7608967-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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