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Article

Characterization of bacteriophage T7 RNA polymerase by linker insertion mutagenesis.

Journal of molecular biology - 20 Nov 1992

Gross L, Chen W J, McAllister W T

Abstract excerpt

Thirty-four mutants of phage T7 RNA polymerase (RNAP) were generated by linker-insertion mutagenesis and characterized with respect to their ability to carry out various steps in the transcription cycle. A number of mutants with interesting biochemical properties were identified. These include: (1) Mutant RNAPs that are catalytically active but that bind weakly to a T7 promoter; one of these mutants is affected...

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Identifiers

PMID: 1453459
DOI: 10.1016/0022-2836(92)90837-a

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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Characterization of bacteriophage T7 RNA polymerase by linker insertion mutagenesis.PMID 1453459Read ↗

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