Article
Characterization of bacteriophage T7 RNA polymerase by linker insertion mutagenesis.
Journal of molecular biology - 20 Nov 1992
Gross L, Chen W J, McAllister W T
Abstract excerpt
Thirty-four mutants of phage T7 RNA polymerase (RNAP) were generated by linker-insertion mutagenesis and characterized with respect to their ability to carry out various steps in the transcription cycle. A number of mutants with interesting biochemical properties were identified. These include: (...
Topics
- Amino Acid Sequence
- Bacteriophage T7
- Base Sequence
- Catalysis
- DNA-Directed RNA Polymerases
- Dimethyl Sulfoxide
- Genetic Complementation Test
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Insertional
- Phenotype
- Promoter Regions, Genetic
- RNA, Viral
- Solubility
- Terminator Regions, Genetic
- Transcription, Genetic
- Viral Proteins