Characterization of a set of T7 RNA polymerase active site mutants.

Article

Characterization of a set of T7 RNA polymerase active site mutants.

The Journal of biological chemistry - 7 Oct 1994

Bonner G, Lafer E M, Sousa R

Abstract excerpt

We have evaluated the elongation rates, processivities, and abortive transcription characteristics of a set of T7 RNA polymerase mutants that map to the polymerase active site. The effects of these mutations on transcription are complex: they cause decreases in activity and processivity during bo...

Topics

Binding Sites
DNA-Directed RNA Polymerases
Mutation
Poly A
Poly C
Poly G
Poly T
Structure-Activity Relationship
Transcription, Genetic
Viral Proteins

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