Article
Cellular and molecular studies of B cells exhibiting reverse somatic mutation throughout life.
Genes to cells : devoted to molecular & cellular mechanisms - 1 Nov 2004
Kodera Takao, Radu Dorel, McGaha Tracy, Zwolo Patty, Stoica Cristina, Cheroute Hilde, Pollock Roberta R, Bona Constantin
Abstract excerpt
Somatic mutation of immunoglobulin (Ig) genes plays an important role in generating antibody diversity. The frequency of somatic mutation appears to vary throughout life. However, this process has been difficult to study in vivo because the DNA in and around rearranged V genes undergoes random mutation, causing silent or replacement mutations. Therefore, we have developed a transgenic mouse model for studying the...
Topics
- Animals
- B-Lymphocytes
- Base Sequence
- Codon, Terminator
- DNA Primers
- Humans
- Immunoglobulin G
- Mice
- Molecular Sequence Data
- Mutation
- Transgenes