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Article

Methods for detecting somatic mutations in vitro: the human T-cell cloning assay selecting for HPRT mutants.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2005

Hou Sai-Mei

Abstract excerpt

The T-cell cloning assay, which detects mutations in the gene for hypoxanthine-guanine phosphoribosyltransferase (HPRT), is the most well-developed reporter system for studying specific locus mutation in human somatic cells. The assay is based on a mitogen- and growth factor-dependent clonal expansion of peripheral T lymphocytes in which the 6-thioguanine-resistant HPRT mutants can be selected, enumerated, and...

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PMID: 15502220
DOI: 10.1385/1-59259-840-4:155

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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