Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

In vitro induction, expression and selection of thioguanine-resistant mutants with human T-lymphocytes.

Mutation research - 1 Feb 1990

O'Neill J P, Sullivan L M, Albertini R J

Abstract excerpt

Conditions have been defined to measure the in vitro induction of mutations at the hypoxanthine-guanine phosphoribosyl transferase (hprt) locus in human T-lymphocytes by a cell cloning assay. The in vitro growth of mass cultures as well as cell cloning is accomplished by the use of crude T-cell growth factor (TCGF) and irradiated human lymphoblastoid feeder cells. These initial studies employed irradiation of G0...

Topics

Cells, Cultured
Clone Cells
Drug Resistance
Gamma Rays
Gene Frequency
Humans
Hypoxanthine Phosphoribosyltransferase
Interleukin-2
Mutagenicity Tests
Mutation
Phytohemagglutinins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

T-Lymphocytes

Thioguanine

Source

Identifiers

PMID: 2300074
DOI: 10.1016/0165-1218(90)90017-v

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2300074-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

In vitro induction, expression and selection of thioguanine-resistant mutants with human T-lymphocytes.PMID 2300074Read ↗

Reduced proliferation rate of hypoxanthine-phosphoribosyl transferase mutant human T-lymphocytes in vitro.Semantic proximity Read ↗

Methods for detecting somatic mutations in vitro: the human T-cell cloning assay selecting for HPRT mutants.Semantic proximity Read ↗

Quantification and molecular characterization of hprt mutants of human T-lymphocytes.Semantic proximity Read ↗

The human T-cell cloning assay: identifying genotypes susceptible to drug toxicity and somatic mutation.Semantic proximity Read ↗

A quantitative assay for measuring the induction of mutations in human peripheral blood T-lymphocytes.Semantic proximity Read ↗

Southern-blot analyses of human T-lymphocyte mutants induced in vitro by gamma-irradiation.Semantic proximity Read ↗

Frequency of HPRT mutant lymphocytes in a human control population as determined by the T-cell cloning procedure.Semantic proximity Read ↗

Somatic gene mutations in vivo as indicated by the 6-thioguanine-resistant T-lymphocytes in human blood.Semantic proximity Read ↗

hprt mutations in vivo in human T-lymphocytes: frequencies, spectra and clonality.Semantic proximity Read ↗

Bromodeoxyuridine labelling as an alternative method to identify 6-thioguanine-resistant mutant lymphocytes in humans.Semantic proximity Read ↗

Molecular analysis of in vivo hprt mutations in human T lymphocytes. V. Effects of total body irradiation secondary to radioimmunoglobulin therapy (RIT)Semantic proximity Read ↗

Alterations of the hprt gene in human in vivo-derived 6-thioguanine-resistant T lymphocytes.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.