Article
The DnaK-DnaJ-GrpE chaperone system activates inert wild type pi initiator protein of R6K into a form active in replication initiation.
The Journal of biological chemistry - 3 Dec 2004
Zzaman Shamsu, Reddy Jagan M, Bastia Deepak
Abstract excerpt
The plasmid R6K is an interesting model system for investigating initiation of DNA replication, not only near the primary binding sites of the initiator protein pi but also at a distance, caused by pi -mediated DNA looping. An important milestone in the mechanistic analysis of this replicon was the development of a reconstituted replication system consisting of 22 different highly purified proteins (Abhyankar, M....
Topics
- ATPases Associated with Diverse Cellular Activities
- Adenosine Triphosphatases
- Adenosine Triphosphate
- Binding Sites
- DNA
- DNA Helicases
- DNA Primers
- DNA Replication
- DNA-Binding Proteins
- Dimerization
- Dose-Response Relationship, Drug
- Electrophoresis, Polyacrylamide Gel