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Article

Reconstitution of R6K DNA replication in vitro using 22 purified proteins.

The Journal of biological chemistry - 14 Nov 2003

Abhyankar Mayuresh M, Zzaman S, Bastia Deepak

Abstract excerpt

We have reconstituted a multiprotein system consisting of 22 purified proteins that catalyzed the initiation of replication specifically at ori gamma of R6K, elongation of the forks, and their termination at specific replication terminators. The initiation was strictly dependent on the plasmid-encoded initiator protein pi and on the host-encoded initiator DnaA. The wild type pi was almost inert, whereas a mutant...

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Identifiers

PMID: 12970346
DOI: 10.1074/jbc.M308516200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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Reconstitution of R6K DNA replication in vitro using 22 purified proteins.PMID 12970346Read ↗

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