Article
Chaperone-mediated reduction of RepA dimerization is associated with RepA conformational change.
Molecular microbiology - 1 Oct 1997
Dibbens J A, Muraiso K, Chattoraj D K
Abstract excerpt
RepA, the initiator protein of plasmid P1, binds to multiple sites (iterons) in the origin. The binding normally requires participation of chaperones, DnaJ, DnaK and GrpE. When purified, RepA appears dimeric and is inactive in iteron binding. On reaction with chaperones, a species active in itero...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Binding Sites
- DNA Footprinting
- DNA Helicases
- DNA, Recombinant
- DNA-Binding Proteins
- Dimerization
- HSP40 Heat-Shock Proteins
- Heat-Shock Proteins
- Models, Chemical
- Molecular Chaperones
- Molecular Sequence Data
- Mutation
- Plasmids
- Protein Conformation
- Protein Folding
- Proteins