The requirement for molecular chaperones in lambda DNA replication is reduced by the mutation pi in lambda P gene, which weakens the interaction between lambda P protein and DnaB helicase.

Abstract excerpt

During the initiation of lambda DNA replication, the host DnaB helicase is complexed with phage lambda P protein in order to be properly positioned near the ori lambda-lambda O initiation complex. However, the lambda P-DnaB interaction inhibits the activities of DnaB. Thus, the concerted action of bacterial heat shock proteins, DnaK, DnaJ, and GrpE, is required to activate the helicase. Wild-type phage lambda...

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