Article
The requirement for molecular chaperones in lambda DNA replication is reduced by the mutation pi in lambda P gene, which weakens the interaction between lambda P protein and DnaB helicase.
The Journal of biological chemistry - 28 Apr 1995
Konieczny I, Marszalek J
Abstract excerpt
During the initiation of lambda DNA replication, the host DnaB helicase is complexed with phage lambda P protein in order to be properly positioned near the ori lambda-lambda O initiation complex. However, the lambda P-DnaB interaction inhibits the activities of DnaB. Thus, the concerted action of bacterial heat shock proteins, DnaK, DnaJ, and GrpE, is required to activate the helicase. Wild-type phage lambda...
Topics
- Bacterial Proteins
- Bacteriophage M13
- Bacteriophage lambda
- Chaperonins
- Cloning, Molecular
- DNA Helicases
- DNA Replication
- DNA, Single-Stranded
- DNA, Viral
- DnaB Helicases
- Escherichia coli Proteins