Replication of the R6K gamma origin in vitro: dependence on wt pi and hyperactive piS87N protein variant.

doi:10.1016/s0378-1119(97)00092-9

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Article

Replication of the R6K gamma origin in vitro: dependence on wt pi and hyperactive piS87N protein variant.

Gene - 1 Jul 1997

Levchenko I, Inman R B, Filutowicz M

Abstract excerpt

The pi protein of plasmid R6K is involved in control of replication. The aim of this study was to use an in vitro replication system dependent on an R6K-derived gamma origin of replication (gamma ori) to compare replication characteristics of wt pi and a hyperactive variant of pi protein (piS87N;...

Topics

Cell-Free System
DNA Helicases
DNA Replication
DNA-Binding Proteins
DNA-Directed RNA Polymerases
Escherichia coli
Genetic Variation
Plasmids
Replication Origin
Trans-Activators

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