Activation of distant replication origins in vivo by DNA looping as revealed by a novel mutant form of an initiator protein defective in cooperativity at a distance.

doi:10.1002/j.1460-2075.1992.tb05161.x

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Article

Activation of distant replication origins in vivo by DNA looping as revealed by a novel mutant form of an initiator protein defective in cooperativity at a distance.

The EMBO journal - 1 Mar 1992

Miron A, Mukherjee S, Bastia D

Abstract excerpt

We have isolated mutants of the pi initiator protein of the plasmid R6K that are defective in DNA looping in vitro but retain their normal DNA binding affinity for the primary binding sites (iterons) at the gamma origin/enhancer. One such looping defective mutant called R6 was determined to be a proline to leucine change at position 46 near the N terminus of the pi protein. Using a set of genetic assays that...

Topics

Autoradiography
Bacterial Proteins
Base Sequence
Carrier Proteins
DNA Fingerprinting
DNA Helicases
DNA Replication
DNA, Bacterial
DNA-Binding Proteins
Electrophoresis, Agar Gel
Electrophoresis, Polyacrylamide Gel

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Enhancer Elements, Genetic

Escherichia coli

Escherichia coli Proteins

Kinetics

Lipoproteins

Molecular Sequence Data

Mutation

Source

Open in PubMed Open full text

Identifiers

PMID: 1547780
DOI: 10.1002/j.1460-2075.1992.tb05161.x
PMCID: PMC556563

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1547780-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Activation of distant replication origins in vivo by DNA looping as revealed by a novel mutant form of an initiator protein defective in cooperativity at a distance.PMID 1547780Read ↗

Replication initiation at a distance: determination of the cis- and trans-acting elements of replication origin alpha of plasmid R6K.Semantic proximity Read ↗

Binding modes of the initiator and inhibitor forms of the replication protein pi to the gamma ori iteron of plasmid R6K.Semantic proximity Read ↗

Replication of the R6K gamma origin in vitro: dependence on wt pi and hyperactive piS87N protein variant.Semantic proximity Read ↗

The DnaK-DnaJ-GrpE chaperone system activates inert wild type pi initiator protein of R6K into a form active in replication initiation.Semantic proximity Read ↗

Cooperative binding mode of the inhibitors of R6K replication, pi dimers.Semantic proximity Read ↗

Mechanism of origin activation by monomers of R6K-encoded pi protein.Semantic proximity Read ↗

Cooperative binding of initiator protein to replication origin conferred by single amino acid substitution.Semantic proximity Read ↗

Identification of a novel promoter in the replication control region of plasmid R6K.Semantic proximity Read ↗

Multiple pathways of copy control of gamma replicon of R6K: mechanisms both dependent on and independent of cooperativity of interaction of tau protein with DNA affect the copy number.Semantic proximity Read ↗

Altered (copy-up) forms of initiator protein pi suppress the point mutations inactivating the gamma origin of plasmid R6K.Semantic proximity Read ↗

Autoregulation-deficient mutant of the plasmid R6K-encoded pi protein distinguishes between palindromic and nonpalindromic binding sites.Semantic proximity Read ↗

Biochemical investigations of control of replication initiation of plasmid R6K.Semantic proximity Read ↗

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