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Article

Biochemical investigations of control of replication initiation of plasmid R6K.

The Journal of biological chemistry - 20 Feb 2004

Abhyankar Mayuresh M, Reddy Jagan M, Sharma Rahul, Büllesbach Erika, Bastia Deepak

Abstract excerpt

The mechanistic basis of control of replication initiation of plasmid R6K was investigated by addressing the following questions. What are the biochemical attributes of mutations in the pi initiator protein that caused loss of negative control of initiation? Did the primary control involve only initiator protein-ori DNA interaction or did it also involve protein-protein interactions between pi and several...

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PMID: 14665626
DOI: 10.1074/jbc.M312052200

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Biochemical investigations of control of replication initiation of plasmid R6K.PMID 14665626Read ↗

The DnaK-DnaJ-GrpE chaperone system activates inert wild type pi initiator protein of R6K into a form active in replication initiation.Semantic proximity Read ↗

Binding modes of the initiator and inhibitor forms of the replication protein pi to the gamma ori iteron of plasmid R6K.Semantic proximity Read ↗

N-terminal truncated forms of the bifunctional pi initiation protein express negative activity on plasmid R6K replication.Semantic proximity Read ↗

Replication of the R6K gamma origin in vitro: dependence on wt pi and hyperactive piS87N protein variant.Semantic proximity Read ↗

Mechanism of origin activation by monomers of R6K-encoded pi protein.Semantic proximity Read ↗

Cooperative binding mode of the inhibitors of R6K replication, pi dimers.Semantic proximity Read ↗

Altered (copy-up) forms of initiator protein pi suppress the point mutations inactivating the gamma origin of plasmid R6K.Semantic proximity Read ↗

Reconstitution of R6K DNA replication in vitro using 22 purified proteins.Semantic proximity Read ↗

Identification of a novel promoter in the replication control region of plasmid R6K.Semantic proximity Read ↗

Structure-based functional analysis of the replication protein of plasmid R6K: key amino acids at the pi/DNA interface.Semantic proximity Read ↗

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Autoregulation-deficient mutant of the plasmid R6K-encoded pi protein distinguishes between palindromic and nonpalindromic binding sites.Semantic proximity Read ↗

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