Article
Targeting and germ-line transmission of a null mutation at the metallothionein I and II loci in mouse.
Proceedings of the National Academy of Sciences of the United States of America - 1 Sept 1993
Michalska A E, Choo K H
Abstract excerpt
We report the generation of transgenic mice deficient in the metallothionein MT-I and MT-II genes. The mutations were introduced into embryonic stem cells by homologous recombination. Chimeric mice resulting from the targeted embryonic stem cells transmitted the disrupted alleles through their ge...
Topics
- Animals
- Base Sequence
- Blotting, Southern
- Chimera
- Crosses, Genetic
- DNA
- Embryo, Mammalian
- Female
- Genetic Carrier Screening
- Homozygote
- Male
- Metallothionein
- Mice
- Mice, Inbred C57BL
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Polymerase Chain Reaction