Article
Selection of glucocorticoid-resistant mutations from an AtT-20 cell line containing a glucocorticoid-regulated selectable transgene.
Biochemical and biophysical research communications - 6 Apr 1995
Harrison R W, Lippman S S, VerHoeven R
Abstract excerpt
AtT-20/IDG8 cells contain the stably transfected, selectable gene, neomycin phosphotransferase, under negative glucocorticoid regulation. Thus, when cultured in the simultaneous presence of the neomycin analogue, G418, and dexamethasone, AtT-20/IDG8 cells fail to grow. Our hypothesis was that mutated AtT-20/IDG8 cells capable of growth in such medium would have a defect in the glucocorticoid-mediated regulation...
Topics
- Animals
- Cell Division
- Dexamethasone
- Drug Resistance
- Gene Expression Regulation
- Gentamicins
- Kanamycin Kinase
- Mice
- Mutation
- Phosphotransferases (Alcohol Group Acceptor)
- RNA, Messenger