Article
Characterization of synthetic DNA bar codes in Saccharomyces cerevisiae gene-deletion strains.
Proceedings of the National Academy of Sciences of the United States of America - 27 Jul 2004
Eason Robert G, Pourmand Nader, Tongprasit Waraporn, Herman Zelek S, Anthony Kevin, Jejelowo Olufisayo, Davis Ronald W, Stolc Viktor
Abstract excerpt
Incorporation of strain-specific synthetic DNA tags into yeast Saccharomyces cerevisiae gene-deletion strains has enabled identification of gene functions by massively parallel growth rate analysis. However, it is important to confirm the sequences of these tags, because mutations introduced during construction could lead to significant errors in hybridization performance. To validate this experimental system, we...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.