Article
Quantitative phenotyping via deep barcode sequencing.
Genome research - 1 Oct 2009
Smith Andrew M, Heisler Lawrence E, Mellor Joseph, Kaper Fiona, Thompson Michael J, Chee Mark, Roth Frederick P, Giaever Guri, Nislow Corey
Abstract excerpt
Next-generation DNA sequencing technologies have revolutionized diverse genomics applications, including de novo genome sequencing, SNP detection, chromatin immunoprecipitation, and transcriptome analysis. Here we apply deep sequencing to genome-scale fitness profiling to evaluate yeast strain collections in parallel. This method, Barcode analysis by Sequencing, or "Bar-seq," outperforms the current benchmark...
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