Article
Analysis of genetic interactions on a genome-wide scale in budding yeast: diploid-based synthetic lethality analysis by microarray.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2008
Meluh Pamela B, Pan Xuewen, Yuan Daniel S, Tiffany Carol, Chen Ou, Sookhai-Mahadeo Sharon, Wang Xiaoling, Peyser Brian D, Irizarry Rafael, Spencer Forrest A, Boeke Jef D
Abstract excerpt
Comprehensive collections of open reading frame (ORF) deletion mutant strains exist for the budding yeast Saccharomyces cerevisiae. With great prescience, these strains were designed with short molecular bar codes or TAGs that uniquely mark each deletion allele, flanked by shared priming sequences. These features have enabled researchers to handle yeast mutant collections as complex pools of approximately 6000...
Topics
- Gene Deletion
- Gene Expression Profiling
- Gene Expression Regulation, Fungal
- Genes, Lethal
- Genome, Fungal
- Mutation
- Oligonucleotide Array Sequence Analysis
- Saccharomyces cerevisiae