Article
Functional profiling of the Saccharomyces cerevisiae genome.
Nature - 25 Jul 2002
Giaever Guri, Chu Angela M, Ni Li, Connelly Carla, Riles Linda, Véronneau Steeve, Dow Sally, Lucau-Danila Ankuta, Anderson Keith, André Bruno, Arkin Adam P, Astromoff Anna, El-Bakkoury Mohamed, Bangham Rhonda, Benito Rocio, Brachat Sophie, Campanaro Stefano, Curtiss Matt, Davis Karen, Deutschbauer Adam, Entian Karl-Dieter, Flaherty Patrick, Foury Francoise, Garfinkel David J, Gerstein Mark, Gotte Deanna, Güldener Ulrich, Hegemann Johannes H, Hempel Svenja, Herman Zelek, Jaramillo Daniel F, Kelly Diane E, Kelly Steven L, Kötter Peter, LaBonte Darlene, Lamb David C, Lan Ning, Liang Hong, Liao Hong, Liu Lucy, Luo Chuanyun, Lussier Marc, Mao Rong, Menard Patrice, Ooi Siew Loon, Revuelta Jose L, Roberts Christopher J, Rose Matthias, Ross-Macdonald Petra, Scherens Bart, Schimmack Greg, Shafer Brenda, Shoemaker Daniel D, Sookhai-Mahadeo Sharon, Storms Reginald K, Strathern Jeffrey N, Valle Giorgio, Voet Marleen, Volckaert Guido, Wang Ching-yun, Ward Teresa R, Wilhelmy Julie, Winzeler Elizabeth A, Yang Yonghong, Yen Grace, Youngman Elaine, Yu Kexin, Bussey Howard, Boeke Jef D, Snyder Michael, Philippsen Peter, Davis Ronald W, Johnston Mark
Abstract excerpt
Determining the effect of gene deletion is a fundamental approach to understanding gene function. Conventional genetic screens exhibit biases, and genes contributing to a phenotype are often missed. We systematically constructed a nearly complete collection of gene-deletion mutants (96% of annotated open reading frames, or ORFs) of the yeast Saccharomyces cerevisiae. DNA sequences dubbed 'molecular bar codes'...
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