Article
Quantitative phenotypic analysis of yeast deletion mutants using a highly parallel molecular bar-coding strategy.
Nature genetics - 1 Dec 1996
Shoemaker D D, Lashkari D A, Morris D, Mittmann M, Davis R W
Abstract excerpt
A quantitative and highly parallel method for analysing deletion mutants has been developed to aid in determining the biological function of thousands of newly identified open reading frames (ORFs) in Saccharomyces cerevisiae. This approach uses a PCR targeting strategy to generate large numbers...
Topics
- DNA
- DNA Mutational Analysis
- Fluorescence
- Genetic Techniques
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Open Reading Frames
- Phenotype
- Pilot Projects
- Polymerase Chain Reaction
- Saccharomyces cerevisiae
- Sequence Deletion