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Article

A novel human leucocyte antigen-DRB1 genotyping method based on multiplex primer extension reactions.

Tissue antigens - 1 Jul 2004

Jaakkola E, Herzberg I, Crane A M, Pointon J J, Laiho K, Kauppi M, Kaarela K, Wordsworth B P, Tuomilehto J, Brown M A

Abstract excerpt

We have developed and validated a semi-automated fluorescent method of genotyping human leucocyte antigen (HLA)-DRB1 alleles, HLA-DRB1*01-16, by multiplex primer extension reactions. This method is based on the extension of a primer that anneals immediately adjacent to the single-nucleotide polymorphism with fluorescent dideoxynucleotide triphosphates (minisequencing), followed by analysis on an ABI Prism 3700...

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PMID: 15191530
DOI: 10.1111/j.1399-0039.2004.00241.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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