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High throughput <scp>HLA</scp> genotyping using 454 sequencing and the Fluidigm Access Array™ system for simplified amplicon library preparation

10 Feb 2013

Abstract excerpt

The human leukocyte antigen (HLA) class I and class II loci are the most polymorphic genes in the human genome; distinguishing the thousands of HLA alleles is challenging. Next generation sequencing of exonic amplicons with the 454 genome sequence (GS) FLX System and Conexio Assign ATF 454 software provides high resolution, high throughput HLA genotyping for eight class I and class II loci. HLA typing of...

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PMID: 23398507
DOI: 10.1111/tan.12071

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