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Article

Genotyping of single-nucleotide polymorphisms by primer extension reaction in a dry-reagent dipstick format.

Analytical chemistry - 15 Jan 2007

Litos Ioannis K, Ioannou Penelope C, Christopoulos Theodore K, Traeger-Synodinos Joanne, Kanavakis Emmanuel

Abstract excerpt

The primer extension (PEXT) reaction is the most widely used approach to genotyping of single-nucleotide polymorphisms (SNPs). Current methods for analysis of PEXT reaction products are based on electrophoresis, fluorescence resonance energy transfer, fluorescence polarization, pyrosequencing, mass spectrometry, microarrays, and spectrally encoded microspheres. We report the first dry-reagent dipstick method that...

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DNA Primers
Genotype
Mannose-Binding Lectin
Nucleic Acid Hybridization
Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Polymorphism, Single Nucleotide

Sensitivity and Specificity

Sequence Analysis, DNA

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Identifiers

PMID: 17222001
DOI: 10.1021/ac061729e

Genes

MBL2

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