Article
Sequence-specific priming and exonuclease-released fluorescence detection of HLA-DQB1 alleles.
Tissue antigens - 1 Aug 1996
Faas S J, Menon R, Braun E R, Rudert W A, Trucco M
Abstract excerpt
Molecular typing of HLA DQB1 alleles, employing sequence-specific primers (SSP) for PCR amplification, was used to test a novel method that eliminates the requirement for subsequent gel electrophoresis or additional hybridization steps by directly detecting positive reactions. We have evaluated the performance of this fluorescence-based oligonucleotide probe assay to assign the most common DQB1 alleles on DNA...
Topics
- Alleles
- Base Sequence
- Cell Line
- DNA Primers
- Exonucleases
- Fluorescent Dyes
- Genetic Carrier Screening
- HLA-DQ Antigens
- HLA-DQ beta-Chains
- HLA-DR Antigens
- HLA-DRB1 Chains