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Article

Multiplex single nucleotide extension: a robust and high throughput method for HLA-A locus typing.

Human immunology - 1 Dec 2003

Han Mei, Tan Yue Qing, Zhang Yanzheng, Tsai Jeannette, Vorhaben Rob, Moraes J Roberto, Moraes M Elisa, Stastny Peter

Abstract excerpt

This report describes a typing method that can identify all known human leukocyte antigen A (HLA-A) alleles by determining nucleotides present at polymorphic sites using single nucleotide extension. Allele specific primers are bound to capture oligonucleotides which allows for a multiplex approach during single nucleotide extension (SNE) reactions. Eleven group-specific polymerase chain reaction amplifications...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 14630394
DOI: 10.1016/j.humimm.2003.08.358

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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