Article
Multiplex single nucleotide extension: a robust and high throughput method for HLA-A locus typing.
Human immunology - 1 Dec 2003
Han Mei, Tan Yue Qing, Zhang Yanzheng, Tsai Jeannette, Vorhaben Rob, Moraes J Roberto, Moraes M Elisa, Stastny Peter
Abstract excerpt
This report describes a typing method that can identify all known human leukocyte antigen A (HLA-A) alleles by determining nucleotides present at polymorphic sites using single nucleotide extension. Allele specific primers are bound to capture oligonucleotides which allows for a multiplex approac...
Topics
- Alleles
- Base Sequence
- HLA-A Antigens
- Histocompatibility Testing
- Humans
- Leukocytes
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Polymorphism, Single-Stranded Conformational
- Reproducibility of Results