Genotyping of single nucleotide polymorphisms by primer extension reaction and a dual-analyte bio/chemiluminometric assay.

doi:10.1007/s00216-007-1383-z

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Article

Genotyping of single nucleotide polymorphisms by primer extension reaction and a dual-analyte bio/chemiluminometric assay.

Analytical and bioanalytical chemistry - 1 Aug 2007

Konstantou Jessica, Ioannou Penelope C, Christopoulos Theodore K

Abstract excerpt

Primer extension reaction (PEXT) is the most widely used approach to genotyping of single nucleotide polymorphisms (SNP). It is based on the high accuracy of nucleotide incorporation by the DNA polymerase. We propose a dual-analyte bio/chemiluminometric method for the simultaneous detection of the PEXT reaction products of the normal and mutant allele in a high sample-throughput format. PCR-amplified DNA...

Topics

Cytochrome P-450 CYP2D6
DNA Mutational Analysis
Genotype
Humans
Luminescence
Mannose-Binding Lectin
Methods
Nucleic Acid Hybridization
Polymorphism, Single Nucleotide

Source

Open in PubMed

Identifiers

PMID: 17554527
DOI: 10.1007/s00216-007-1383-z

Genes

MBL2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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