Article
Multiplexed, ligation-dependent probe amplification for rapid and inexpensive HLA-DQB1 allelotyping.
Tissue antigens - 1 Oct 2011
Akers N K, Curry J D, Smith M T, Bracci P M, Skibola C F
Abstract excerpt
Many effective options exist to accurately type DNA for human leukocyte antigen (HLA) alleles. However, most of the existing methods are excessively costly in terms of overall monetary costs, DNA requirements, and proprietary software. We present a novel assay capable of resolving heterozygous HLA-DQB1 allelotypes at two digits, with even greater specificity for the HLA-DQB1*06 allele family, by using the...
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