Article
High-throughput DNA typing of HLA-A, -B, -C, and -DRB1 loci by a PCR-SSOP-Luminex method in the Japanese population.
Immunogenetics - 1 Nov 2005
Itoh Yoshiki, Mizuki Nobuhisa, Shimada Tsuyako, Azuma Fumihiro, Itakura Mitsuo, Kashiwase Koichi, Kikkawa Eri, Kulski Jerzy K, Satake Masahiro, Inoko Hidetoshi
Abstract excerpt
We have developed a new high-throughput, high-resolution genotyping method for the detection of alleles at the human leukocyte antigen (HLA)-A, -B, -C, and -DRB1 loci by combining polymerase chain reaction (PCR) and sequence-specific oligonucleotide probes (SSOPs) protocols with the Luminex 100 xMAP flow cytometry dual-laser system to quantitate fluorescently labeled oligonucleotides attached to color-coded...
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