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Article

Cost-efficient multiplex PCR for routine genotyping of up to nine classical HLA loci in a single analytical run of multiple samples by next generation sequencing.

BMC genomics - 18 Apr 2015

Ozaki Yuki, Suzuki Shingo, Kashiwase Koichi, Shigenari Atsuko, Okudaira Yuko, Ito Sayaka, Masuya Anri, Azuma Fumihiro, Yabe Toshio, Morishima Satoko, Mitsunaga Shigeki, Satake Masahiro, Ota Masao, Morishima Yasuo, Kulski Jerzy K, Saito Katsuyuki, Inoko Hidetoshi, Shiina Takashi

Abstract excerpt

BACKGROUND: HLA genotyping by next generation sequencing (NGS) requires three basic steps, PCR, NGS, and allele assignment. Compared to the conventional methods, such as PCR-sequence specific oligonucleotide primers (SSOP) and -sequence based typing (SBT), PCR-NGS is extremely labor intensive and time consuming. In order to simplify and accelerate the NGS-based HLA genotyping method for multiple DNA samples, we...

Topics

Alleles
DNA Primers
Genetic Loci
Genotype
Genotyping Techniques
HLA Antigens
High-Throughput Nucleotide Sequencing
Humans
Multiplex Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Sequence Analysis, DNA

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Identifiers

PMID: 25895492
DOI: 10.1186/s12864-015-1514-4
PMCID: PMC4404632

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-25895492-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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