Article
Oncogenic signal-induced ability to enter S phase in the absence of anchorage is the mechanism for the growth of transformed NRK cells in soft agar.
The New biologist - 1 May 1992
Kume K, Jinno S, Miwatani H, Kizaka-Kondoh S, Terada Y, Nojima H, Okayama H
Abstract excerpt
Upon neoplastic transformation, cells acquire the ability to grow in soft agar. We investigated how this occurs by cell cycle analysis of a rat cell line NRK-49F and its transformation-deficient mutants. Rapidly growing NRK and mutants arrest in G1 when deprived of anchorage by suspending in methylcellulose. Addition of epidermal growth factor (EGF) together with transforming growth factor-beta (TGF-beta), which...
Topics
- Agar
- Animals
- Cell Adhesion
- Cell Division
- Cell Line
- Cell Transformation, Neoplastic
- Gene Expression
- Genes, fos
- Genes, myc
- Mutation
- Oncogenes