Article
Altered response to growth factors or retinoic acid in phenotypic transformation of normal rat kidney cells expressing human c-fos gene.
Biochemical and biophysical research communications - 31 Dec 1991
Sato S, Kohno K, Ono M, Sato Y, Kuwano M
Abstract excerpt
Anchorage-independent growth of normal rat kidney (NRK) fibroblast in soft agar depends on both transforming growth factor beta (TGF beta) and epidermal growth factor (EGF). To examine whether c-fos protein is involved in phenotypic transformation of NRK cells, we have transfected and isolated several NRK cell lines that carry the human c-fos gene fused to the metallothionein IIA promoter. A transfectant, Nf-1,...
Topics
- Animals
- Blotting, Northern
- Blotting, Southern
- Blotting, Western
- Cell Line
- Cell Transformation, Neoplastic
- Epidermal Growth Factor
- Fibroblast Growth Factor 2
- Genes, fos
- Growth Substances