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Article

Ricin A-chain substrate specificity in RNA, DNA, and hybrid stem-loop structures.

Biochemistry - 4 May 2004

Amukele Tim K, Schramm Vern L

Abstract excerpt

Ricin toxin A-chain (RTA) is the catalytic subunit of ricin, a heterodimeric toxin from castor beans. Its ribosomal inactivating activity arises from depurination of a single adenine from position A(4324) in a GAGA tetraloop from 28S ribosomal RNA. Minimal substrate requirements are the GAGA tetraloop and stem of two or more base pairs. Depurination activity also occurs on stem-loop DNA with the same sequence,...

Topics

Base Pairing
Base Sequence
Catalysis
DNA
Hydrogen Bonding
Kinetics
Methylation
Models, Molecular
Mutation
Oligonucleotides
RNA
RNA, Ribosomal, 28S

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Ricin

Substrate Specificity

Source

Identifiers

PMID: 15109249
DOI: 10.1021/bi0498508

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