Article
The ribotoxin restrictocin recognizes its RNA substrate by selective engagement of active site residues.
Biochemistry - 12 Apr 2011
Plantinga Matthew J, Korennykh Alexei V, Piccirilli Joseph A, Correll Carl C
Abstract excerpt
Restrictocin and related fungal endoribonucleases from the α-sarcin family site-specifically cleave the sarcin/ricin loop (SRL) on the ribosome to inhibit translation and ultimately trigger cell death. Previous studies showed that the SRL folds into a bulged-G motif and tetraloop, with restrictocin achieving a specificity of ∼1000-fold by recognizing both motifs only after the initial binding step. Here, we...
Topics
- Algorithms
- Amino Acid Substitution
- Animals
- Base Sequence
- Binding Sites
- Biocatalysis
- Catalytic Domain
- Fungal Proteins
- Kinetics
- Models, Molecular
- Mutation
- Nucleic Acid Conformation
- Protein Binding
- Protein Structure, Tertiary