Article
The electrostatic character of the ribosomal surface enables extraordinarily rapid target location by ribotoxins.
Nature structural & molecular biology - 1 May 2006
Korennykh Alexei V, Piccirilli Joseph A, Correll Carl C
Abstract excerpt
Alpha-sarcin ribotoxins comprise a unique family of ribonucleases that cripple the ribosome by catalyzing endoribonucleolytic cleavage of ribosomal RNA at a specific location in the sarcin/ricin loop (SRL). The SRL structure alone is cleaved site-specifically by the ribotoxin, but the ribosomal context enhances the reaction rate by several orders of magnitude. We show that, for the alpha-sarcin-like ribotoxin...
Topics
- Animals
- Binding Sites
- Crystallography, X-Ray
- DNA
- Endoribonucleases
- Fungal Proteins
- Models, Molecular
- Mutation
- Nucleic Acid Conformation
- Protein Conformation
- Rats
- Ribosomes
- Static Electricity
- Substrate Specificity
- Time Factors
- Titrimetry