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Article

Transactivator mutants with altered effector specificity allow selective regulation of two genes by tetracycline variants.

Gene - 28 Apr 2004

Krueger Christel, Schmidt Andreas, Danke Christina, Hillen Wolfgang, Berens Christian

Abstract excerpt

A set of Tet repressor (TetR) based eukaryotic transactivators that respond to 4-de(dimethylamino)-6-deoxy-6-demethyl-tetracycline (cmt3) but no longer to tetracycline (tc) is presented. The novel transactivators exhibit high activation in absence of an effector and a 200-fold reduction of reporter gene activity in the presence of cmt3. The most cmt3-sensitive mutant was coexpressed with a tc-responsive Tet...

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Source

Identifiers

PMID: 15094198
DOI: 10.1016/j.gene.2004.02.006

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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