Stepwise selection of TetR variants recognizing tet operator 6C with high affinity and specificity.

doi:10.1006/jmbi.1997.1539

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Article

Stepwise selection of TetR variants recognizing tet operator 6C with high affinity and specificity.

Journal of molecular biology - 20 Feb 1998

Helbl V, Tiebel B, Hillen W

Abstract excerpt

The exchange of Trp43 to Arg in the sixth position of the TetR recognition alpha-helix leads to a new DNA recognition specificity for tetO-6C, however, it is bound with only low affinity. Specificity and affinity of this mutant were substantially increased by additional amino acid exchanges in th...

Topics

Helix-Turn-Helix Motifs
Models, Molecular
Mutagenesis
Mutation
Operator Regions, Genetic
Protein Conformation
Repressor Proteins
Structure-Activity Relationship
Substrate Specificity

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