Stepwise selection of TetR variants recognizing tet operator 6C with high affinity and specificity.

Abstract excerpt

The exchange of Trp43 to Arg in the sixth position of the TetR recognition alpha-helix leads to a new DNA recognition specificity for tetO-6C, however, it is bound with only low affinity. Specificity and affinity of this mutant were substantially increased by additional amino acid exchanges in the last positions of the recognition alpha-helix and the turn, which most likely play structural roles in the formation...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Models, Molecular
• Share to Mutagenesis
• Share to Mutation