Article
Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity.
Proceedings of the National Academy of Sciences of the United States of America - 5 Jul 2000
Urlinger S, Baron U, Thellmann M, Hasan M T, Bujard H, Hillen W
Abstract excerpt
Regulatory elements that control tetracycline resistance in Escherichia coli were previously converted into highly specific transcription regulation systems that function in a wide variety of eukaryotic cells. One tetracycline repressor (TetR) mutant gave rise to rtTA, a tetracycline-controlled transactivator that requires doxycycline (Dox) for binding to tet operators and thus for the activation of P(tet)...
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