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Article

Two mutations in the tetracycline repressor change the inducer anhydrotetracycline to a corepressor.

Nucleic acids research - 1 Jan 2004

Kamionka Annette, Bogdanska-Urbaniak Joanna, Scholz Oliver, Hillen Wolfgang

Abstract excerpt

We report for the first time the in vitro characterization of a reverse tetracycline repressor (revTetR). The dimeric wild-type repressor (TetR) binds to tet operator tetO in the absence of the inducer anhydrotetracycline (atc) to confer tight repression. We have isolated the revTetR G96E L205S mutant, which, contrary to TetR, binds tetO only in the presence of atc. This reverse acting mutant was overproduced and...

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Identifiers

PMID: 14764926
DOI: 10.1093/nar/gkh200
PMCID: PMC373327

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Source receipt: pubmed, retrieved 7 Sept 2026.

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