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Article

Teaching TetR to recognize a new inducer.

Journal of molecular biology - 30 May 2003

Scholz Oliver, Köstner Martin, Reich Marco, Gastiger Susanne, Hillen Wolfgang

Abstract excerpt

Tet Repressor (TetR) recognizes the inducer tetracycline (tc) with high affinity. The tc analog 4-de(dimethylamino)-6-deoxy-6-demethyl-tetracycline (cmt3) is not an inducer for TetR. Induction specificity for cmt3 was generated by employing a directed evolution approach to screen appropriate TetR mutants in four successive steps. The specificity of the best TetR mutant is more than 20,000-fold increased for cmt3...

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Source

Identifiers

PMID: 12758071
DOI: 10.1016/s0022-2836(03)00427-3

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Teaching TetR to recognize a new inducer.PMID 12758071Read ↗

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