Article
Generation of conditional mutants in higher eukaryotes by switching between the expression of two genes.
Proceedings of the National Academy of Sciences of the United States of America - 2 Feb 1999
Baron U, Schnappinger D, Helbl V, Gossen M, Hillen W, Bujard H
Abstract excerpt
A regulatory system for the in-depth study of gene functions in higher eukaryotic cells has been developed. It is based on the tetracycline-controlled transactivators and reverse tTA, which were remodeled to discriminate efficiently between two different promoters. The system permits one to contr...
Topics
- Bacterial Proteins
- DNA-Binding Proteins
- Doxorubicin
- Gene Expression Regulation, Bacterial
- Genes, Switch
- HeLa Cells
- Humans
- Phenotype
- Promoter Regions, Genetic
- Recombinant Fusion Proteins
- Tetracycline
- Trans-Activators
- Transcriptional Activation
- Transfection