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Article

Generation of conditional mutants in higher eukaryotes by switching between the expression of two genes.

Proceedings of the National Academy of Sciences of the United States of America - 2 Feb 1999

Baron U, Schnappinger D, Helbl V, Gossen M, Hillen W, Bujard H

Abstract excerpt

A regulatory system for the in-depth study of gene functions in higher eukaryotic cells has been developed. It is based on the tetracycline-controlled transactivators and reverse tTA, which were remodeled to discriminate efficiently between two different promoters. The system permits one to control reversibly the activity of two genes, or two alleles of a gene, in a mutually exclusive way, and also allows one to...

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Identifiers

PMID: 9927685
DOI: 10.1073/pnas.96.3.1013
PMCID: PMC15342

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