Article
Noninducible Tet repressor mutations map from the operator binding motif to the C terminus.
Journal of bacteriology - 1 Feb 1993
Hecht B, Müller G, Hillen W
Abstract excerpt
We have developed a new genetic selection system for Tet repressor mutations with a noninducible phenotype for tetracycline (TetRs). Extensive chemical mutagenesis of tetR yielded 93 single-site Tet repressor mutations. They map from residue 23 preceding the alpha-helix-turn-alpha-helix operator...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Escherichia coli
- Genes, Bacterial
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Oligodeoxyribonucleotides
- Operator Regions, Genetic
- Repressor Proteins
- Restriction Mapping
- Sequence Alignment
- Tetracycline Resistance