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Article

Tetracycline-inducible expression systems with reduced basal activity in mammalian cells

1 Jan 1999

Abstract excerpt

We describe a modification of the tetracycline-inducible eukaryotic gene expression system with decreased basal levels of expression in HeLa cells. It employs the tetracycline-inducible transactivator and a tetracycline-regulated repressor fusion acting on the same promoter. To avoid heterodimerization or competition for the same DNA site, each was provided with different DNA recognition and/or protein...

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PMID: 9863002
DOI: 10.1093/nar/27.2.708

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