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Article

Phenotypes of combined tet repressor mutants for effector and operator recognition and allostery.

Journal of molecular microbiology and biotechnology - 1 Jan 2004

Bertram Ralph, Kraft Christine, Wisshak Stefanie, Mueller Judith, Scholz Oliver, Hillen Wolfgang

Abstract excerpt

Tet repressor mutants with a shifted effector specificity, preference for a mutant operator sequence or reversion of activity were combined to construct variants bearing two or three phenotypic alterations. TetR alleles with combinations of altered operator and effector specificities can be created by merging the respective residues in a single polypeptide. The mutations giving rise to revTetR, on the other hand,...

Read the complete abstract on PubMed

Topics

Allosteric Regulation
Escherichia coli
Helix-Turn-Helix Motifs
Operator Regions, Genetic
Phenotype
Recombination, Genetic

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Repressor Proteins

Source

Identifiers

PMID: 15925901
DOI: 10.1159/000084565

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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