Article
Optimization of the Tet-On system for regulated gene expression through viral evolution.
Gene therapy - 1 Oct 2006
Zhou X, Vink M, Klaver B, Berkhout B, Das A T
Abstract excerpt
The ability to control (trans)gene expression is important both for basic biological research and applications such as gene therapy. In vivo use of the inducible tetracycline (Tc)-regulated gene expression system (Tet-On system) is limited by its low sensitivity for the effector doxycycline (dox). We used viral evolution to optimize this Escherichia coli-derived regulatory system for its function in mammalian...
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