Article
[Site-specific mutagenesis of residue Lys-172 of phage T7 RNA polymerase: characterization of transcription properties of mutant proteins].
Molekuliarnaia biologiia - 1 Jan 2000
Liakhov D L, Il'genfrits Kh, Chernov B K, Dragan S M, Rechinskiĭ V O, Pokholok D K, Tunitskaia V L, Kochetkov S N
Abstract excerpt
Lys-172 residue of bacteriophage T7 RNA polymerase (T7RP) was substituted for Leu and Gly and Lys-172, Arg-173 were deleted by the site-directed mutagenesis using synthetic oligonucleotides. The specific activity of all mutant enzymes did not differ significantly from that of the wild-type (w.t.) T7RP while for Gly-172 mutant (G172) it was somewhat lower. Leu-172 (L172) and deletion (DEL172-3) mutants were able...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA-Directed RNA Polymerases
- Lysine
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Sequence Deletion