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Article

Sequential release of promoter contacts during transcription initiation to elongation transition.

Journal of molecular biology - 7 Jul 2006

Bandwar Rajiv P, Tang Guo-Qing, Patel Smita S

Abstract excerpt

Bacteriophage T7 RNA polymerase undergoes major conformational changes as transcription proceeds from initiation to elongation. Using limited trypsin digestion and stopped-flow fluorescence kinetic methods, we have monitored promoter release, initial bubble collapse, and refolding of the 152-205 region (subdomain H), the latter being important for RNA channel formation. The kinetic studies show that the...

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Identifiers

PMID: 16780876
DOI: 10.1016/j.jmb.2006.05.029

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Sequential release of promoter contacts during transcription initiation to elongation transition.PMID 16780876Read ↗

Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase.Semantic proximity Read ↗

Weakening of the T7 promoter-polymerase interaction facilitates promoter release.Semantic proximity Read ↗

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